We only got two more weeks to be done with classes and then finals. I’ve had so many difficulties to get my project done. But once I had all the materials available, I started to have problems getting my samples analyzed. The DNA I’m studying is isolated from the surfaces of leaves and along with it a lot of inhibitors and phenolic compounds. This is the first time it happens, because a year ago it all worked well. However science is unpredictable and that’s something I’ve learned through out all these years doing research. You can never have a one definite plan, because things not always work the way you want. I’ve done DNA extractions twice and the quality of it it’s so poor that everyone in the lab thought that the amplification of 16s rDNA wouldn’t work. Guess what! It did! Now I’m in the process to prepare the samples for the tRFLP experiment.

Sometimes when you are not getting everything done because your experiments aren’t succeeding you get depressed and you just wanna give up. In my case I take it as a challenge  and I just keep repeating everything over and over again until it works. It’s all about perseverance. Next semester I hope to have these results analyzed and apply these findings in more specific studies of specific crops or plants.

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